FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002287247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127428
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG421223.73611441262768Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29510.26174620463568404No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA17390.15424488304352915Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATATATATATATATATATATATATATATATATAT16380.14528643957751625No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA14320.1270147628052523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)