FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002287262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054201
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTAT251092.3818038495505127TruSeq Adapter, Index 13 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20370.19322690834100897No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCACCTCGTAT12890.12227269752163013TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)