FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002287268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293121
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTAT232811.8003728962718881TruSeq Adapter, Index 27 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18460.14275539566676282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)