FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002287294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522752
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTAT211794.051443131733595TruSeq Adapter, Index 7 (97% over 37bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18400.35198334965719885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCACCTCGTAT10020.19167788932419197TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)