FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002287410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002287410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872719
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTAT322793.698670476980563TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCACCTCGTAT17240.19754353921479878TruSeq Adapter, Index 22 (97% over 37bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14660.16798075898427786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCCACCCTAATCCATCTCGTAT13600.1558348105174747TruSeq Adapter, Index 16 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)