FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002293662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320125
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGC82110.6219865543035697No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC59920.45389641132468517No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC56040.42450525518416815No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG51650.39125082852002646RNA PCR Primer, Index 26 (100% over 29bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGC49520.3751159928037118No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCA43030.3259539816305274No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC42230.3198939494366064No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC35820.2713379414828141No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG34300.25982388031436415No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA18150.13748698039958338Clontech Universal Primer Mix Long (96% over 26bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC15710.11900388220812423No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15550.11779187576934003Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGC15130.11461035886753147No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG15120.11453460846510748No Hit
CCAGAGGGGAAAAATATGACAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG14470.10961083230754663Clontech Universal Primer Mix Long (96% over 26bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTG13330.10097528643120915No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG13220.10014203200454502RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)