FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002293670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390253
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG69321.776283590388799TruSeq Adapter, Index 8 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGC58641.5026149702885052No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC28340.7261955705657612No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG27710.7100521969081596RNA PCR Primer, Index 8 (96% over 29bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC27040.6928838471453134No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG26190.6711031049088668RNA PCR Primer, Index 8 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC24970.6398413336989082No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG19760.506338195990806TruSeq Adapter, Index 8 (96% over 29bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGC19400.497113411043605No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG15050.3856472595982606No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC14890.38154735517728244No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAA13750.3523355361778128No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAAT13650.3497730959147015No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTC12590.322611229125721No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT12170.3118489800206533No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG11800.3023679510471412TruSeq Adapter, Index 8 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10970.2810996968633169No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTC10670.2734123760739828No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT10290.2636751030741596No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG10060.25778149046900345TruSeq Adapter, Index 8 (96% over 29bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG9920.25419407410064754No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG9820.2516316338375362No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9240.23676948031149023No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAAT8700.22293230289068885No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA7720.19782038831219748No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAAT7590.1944892159701527No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA7260.1860331631018852No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA6990.17911457439148448No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC6980.17885833036517335No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAAT6370.16322744476019402No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAAT6090.15605261202348222No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG5530.14170294655005855No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG5130.1314531854976131No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA5090.13042820939236854No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT5040.12914698926081286TruSeq Adapter, Index 8 (95% over 21bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA4950.12684079302401263No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC4920.12607206094507922No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAAT4900.12555957289245695No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG4870.12479084081352353No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT4750.12171591249778989No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA4620.11838474015574511No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG4600.11787225210312284No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGA4540.11633478794525602No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4480.1147973237873892No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATC4210.10787873507698853No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTG4110.10531629481387715No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTC4090.10480380676125489No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT3980.10198512247183238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)