FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002293671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160460
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC75464.702729652249782No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58673.6563629565000624TruSeq Adapter, Index 1 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC40812.5433129751963106No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC36132.2516515019319456No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC35512.2130125888071794No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA28711.7892309609871622No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG25251.5736008974199178RNA PCR Primer, Index 1 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG24521.5281066932568865RNA PCR Primer, Index 1 (96% over 29bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG19341.205284806182226No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG18541.1554281440857535TruSeq Adapter, Index 1 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG13970.8706219618596535No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG12570.7833728031908264TruSeq Adapter, Index 1 (96% over 29bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT12230.7621837217998255No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA12130.7559516390377664No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT11690.7285304748847065No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA10380.6468901907017325No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT10310.6425277327682911No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9970.6213386513772903No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG9600.5982799451576717No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC9600.5982799451576717No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG9420.5870621961859654RNA PCR Primer, Index 40 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC8690.541567992022934No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8400.5234949520129627No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT8040.5010594540695501No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7800.4861024554406083No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT7270.4530724168016951No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG6830.4256512526486351No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT6420.400099713324193No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG6170.38451950641904525No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT6060.37766421538078027TruSeq Adapter, Index 1 (95% over 21bp)
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG5660.35273588433254394No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT5570.34712700984669076No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG5560.3465038015704849No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA5460.34027171880842577No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG5260.3278075532843076No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG4830.3010095974074536No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA4700.29290788981677673No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT4280.2667331422161286No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA4190.2611242677302754TruSeq Adapter, Index 1 (96% over 29bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG4180.26050105945406954No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3920.24429764427271594TruSeq Adapter, Index 1 (96% over 29bp)
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC3890.2424280194440982No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT3670.22871743736756822No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA3570.22248535460550914No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG3490.21749968839586187No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG3430.21376043873862646No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT3290.20503552287174376TruSeq Adapter, Index 1 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC3290.20503552287174376No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC3170.19755702355727284No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT3150.19631060700486103No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT3130.19506419045244922TruSeq Adapter, Index 1 (95% over 22bp)
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC3090.19257135734762557TruSeq Adapter, Index 1 (95% over 23bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA2930.18260002492833105No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC2700.16826623457559517No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT2660.16577340147077155No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT2580.16078773526112428No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC2570.16016452698491837No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC2550.15891811043250653No Hit
GAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGC2550.15891811043250653TruSeq Adapter, Index 1 (96% over 26bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC2530.15767169388009472No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG2480.1545556524990652No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG2460.15330923594665338No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC2460.15330923594665338No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT2450.15268602767044745TruSeq Adapter, Index 1 (95% over 22bp)
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT2420.15081640284182973TruSeq Adapter, Index 1 (95% over 24bp)
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC2410.15019319456562383No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA2380.1483235697370061TruSeq Adapter, Index 1 (96% over 29bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC2340.14583073663218246No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2330.14520752835597656No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC2310.14396111180356475No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2260.14084507042253522No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTG2250.14022186214632928TruSeq Adapter, Index 1 (96% over 25bp)
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC2230.13897544559391747No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTG2210.1377290290415057No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA2210.1377290290415057No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2210.1377290290415057No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA2150.1339897793842702No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG2110.1314969462794466TruSeq Adapter, Index 1 (96% over 29bp)
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2100.13087373800324067No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTG2090.13025052972703477No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATG2070.12900411317462296No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA1990.12401844696497569No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG1990.12401844696497569No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG1980.12339523868876978RNA PCR Primer, Index 1 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG1940.12090240558394615No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC1930.12027919730774024No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG1920.11965598903153433No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG1920.11965598903153433No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT1880.1171631559267107No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1880.1171631559267107No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCG1870.1165399476505048No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA1860.1159167393742989No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC1850.115293531098093No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC1800.11217748971706346No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT1800.11217748971706346No Hit
CCCGATGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA1780.11093107316465162No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTG1730.10781503178362209No Hit
GCGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGA1730.10781503178362209No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGC1720.10719182350741618No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC1680.10469899040259255TruSeq Adapter, Index 1 (95% over 23bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC1680.10469899040259255No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT1670.10407578212638664No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC1650.10282936557397483No Hit
GCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAA1640.10220615729776891TruSeq Adapter, Index 1 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC1640.10220615729776891No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1620.1009597407453571TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)