FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002293676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445601
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC129820.5308306628922707No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG70080.2865553293444025TruSeq Adapter, Index 1 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC47020.1922635785641239No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC46870.19165023239686277No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC44240.18089622959755086No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGC33430.13669441581026504No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG33020.13501793628641795RNA PCR Primer, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)