FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002293715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049873
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC61590.586642384364585No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGC16940.161352849344635No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG15330.14601766118378126TruSeq Adapter, Index 12 (96% over 29bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAA13670.13020622494339792No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC13480.12839648224118536No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC11570.1102038056031539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)