FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002293791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944844
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG103771.0982765408892896TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG35950.38048609082557544TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT30050.3180419201476646No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG29970.31719521952830304RNA PCR Primer, Index 3 (96% over 28bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT26900.2847030832603054No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG18670.19759875704349078No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAG17630.18659164899179126No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA17530.18553327321758936No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG17430.18447489744338746No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA16950.17939469372721845No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAG15850.1677525602109978No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC12500.13229697177523483No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA12450.13176778388813393Clontech Universal Primer Mix Short (95% over 21bp)
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTC11380.12044316310417381No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA10900.1153629593880048No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC10810.1144104211912231No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG10660.11282285752992029No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTCCGAGCCCA10370.10975356778473483Clontech Universal Primer Mix Short (95% over 21bp)
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT10320.1092243798976339Clontech Universal Primer Mix Short (95% over 21bp)
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA9620.10181574947822074No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACG9620.10181574947822074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)