FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002293793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216922
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90590.7444191164265253TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG40370.3317385995158276TruSeq Adapter, Index 8 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG14900.1224400577851333RNA PCR Primer, Index 8 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)