FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002293835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401079
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58251.452332333530302RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG19060.47521809917746877RNA PCR Primer, Index 41 (96% over 29bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG16590.41363422168699926No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA15710.3916934070345244No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG13700.34157859174875776RNA PCR Primer, Index 41 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA10670.26603237766125876No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA10540.262791120951234No Hit
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGG7290.18175970320061632No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT7000.1745292074628689No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT6510.16231216294046807Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT5650.14087000316645848No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT5390.13438748974640904Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5190.12940094096175567No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA4670.11643591412165684Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACA4620.11518927692549348Clontech Universal Primer Mix Short (95% over 21bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG4560.11369331229009746RNA PCR Primer, Index 41 (96% over 29bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTAA4210.10496685191695401No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC4200.10471752447772135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)