FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002293838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT99741.55898290954564No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT1887.83659858274281No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT893.7098791162984575No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC863.58482701125469No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG723.0012505210504377No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGGCTCTT512.12588578574406No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG381.5839933305543976No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTTCGCTGCTTGGCCTTGTCGCGGCTCTT251.0421008753647354No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT180.7503126302626094No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCNACAACTCCTGTT170.70862859524802No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCT140.5835764902042517No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCGCTT110.45852438516048355No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACTTTGCGCTGCTTGGCCTTGTCGCGGCTCTT100.41684035014589416No Hit
GTCATTTTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT90.3751563151313047No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGNGAAGATGCCGAT80.3334722801167153No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCTTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT80.3334722801167153No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCTCGGCTCTT70.29178824510212586No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCCCTT70.29178824510212586No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGTCCTTGTCGCGGCTCTT60.25010421008753647No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGTCTCTT60.25010421008753647No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTT60.25010421008753647Illumina PCR Primer Index 2 (95% over 21bp)
GTCATTGTCACTGTTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT50.20842017507294708No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTACGCTGCTTGGCCTTGTCGCGGCTCTT50.20842017507294708No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT50.20842017507294708No Hit
GTCATTATCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT50.20842017507294708No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCATTGTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTGGGCCTTGTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGTCTTGTCGCGGCTCTT40.16673614005835766No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCACTTTCAGCTCCATTGGGATCATG40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTATCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNTTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGTGCTGCTTGGCCTTGTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGTGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTTTCGCGGCTCTT40.16673614005835766No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGTCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCACTGCTTGGCCTTGTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGATCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT40.16673614005835766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTC30.12505210504376824No Hit
TTTCAGGTCGGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGGCGCGGCTCTT30.12505210504376824No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGNGGCCCGCGCCCC30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCACGGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCATCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCAACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCNATTGGGATCATG30.12505210504376824No Hit
GTCATTGTCACTGGTCAACTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCATCACCCTTTCAACAACTCCTGTT30.12505210504376824No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTTCAATCAGCACCCTTTCAACAACTCCTGTT30.12505210504376824No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCACTCAGCACCCTTTCAACAACTCCTGTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTTGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
CCTTTAACAAGTCTGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCGTTGTCGCGGCTCTT30.12505210504376824No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTTTT30.12505210504376824No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACCACTCCTGTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGACTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGAGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCTGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTTGCGGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGACTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTGCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTGGCGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCACACACTCGGAAGAAGTAGG30.12505210504376824No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCT30.12505210504376824Illumina PCR Primer Index 2 (95% over 23bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTACTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
GTCATTGTCACTGGTCATCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT30.12505210504376824No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG30.12505210504376824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)