FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002293841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT1420.0RNA PCR Primer, Index 45 (95% over 23bp)
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG34.285714285714286No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT22.857142857142857No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT22.857142857142857No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCCTTCAGCTCGTATGCCGTC11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACCCATCTCCGAGCCCACGAGACGTTCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (100% over 23bp)
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAAGGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCGACGTGCATTCAAATCGTATGCCGTCTT11.4285714285714286No Hit
GCCGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTACAGAATGGCCGGGATGCTGGTGGC11.4285714285714286No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCGGGCAGGTCGAGAAGGGGCCCTG11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCACGAGACGTGCATTCATATCGTATGCCGTCTTC11.4285714285714286RNA PCR Primer, Index 45 (95% over 23bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATATCGTATGCCGTCTT11.4285714285714286No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATTCACATCTCCGAGCCCACTAGCCGTGCATTCAGCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 22bp)
CAACCAAAAGACCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTNGTATACCTTCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGACGTGCAATCAGCTCGTATGCCGTCTT11.4285714285714286No Hit
AAACTCCCTAACCAATCTGTCTCTTATACACATCTCCGAGCCCACTAAACTTGCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (100% over 22bp)
CAACCAAAAGAACAGCCTGTCCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTC11.4285714285714286RNA PCR Primer, Index 45 (100% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACGCATCTGCGAGCCCACGAGGCGTGCATTCAGCGCGTATGCCGTCTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGATACGTGCATTCATCTCGTATGCCGTCTTCT11.4285714285714286RNA PCR Primer, Index 45 (100% over 24bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAACTCGTATGACGTCTTCT11.4285714285714286No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCTAGCCCACGAAACGTGCATTCATCTCGTATGCCATCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 22bp)
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTNGTATGCCGTCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCCACATCTCCGAACCCACGCGCCGTACATTCAGAGAGTATGCCGTCTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGAATTCAGCTCGTATGCAGTCTTCT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCGTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAACGAACGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTCATTCAGCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 23bp)
CAACCAAAAGAACAGCCTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCT11.4285714285714286RNA PCR Primer, Index 45 (96% over 25bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATATCGTATGCCGTCTT11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (100% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAGCTCGTATGCCGTCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACGTGCATTCATCTCGTATGCCTTCTT11.4285714285714286No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT11.4285714285714286No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGNATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 7 (95% over 21bp)
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGACGTGCGTTCAGAACGTATGCCGTCTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCT11.4285714285714286RNA PCR Primer, Index 45 (96% over 25bp)
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTGGTGCTTCCGGAGCTGC11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCACTTATACACATCTCCGAGCACACGAGACGTGCATTCAACTCGTATGCCGTCTTCT11.4285714285714286RNA PCR Primer, Index 45 (95% over 24bp)
CAAGCAATCAAGGCCATTCTCTTATACACATCTCCGAGCCCACGATACGTGCATTCATCTCGNATTCCGTCTTCT11.4285714285714286No Hit
CCTTTAACCAGTATGCCTTTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT11.4285714285714286No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCAAGCCCACGAGACATGCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGATCCCACGATACGTGCATTCAACTCGTATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 7 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGACGTCTT11.4285714285714286No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA11.4285714285714286RNA PCR Primer, Index 45 (96% over 30bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAACTNGTATGCCGGCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTNGTATCCCGTCTT11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 45 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAGCTCGTATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)