FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002293869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172253
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC41842.428985271664354No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39612.299524536582817Illumina PCR Primer Index 6 (96% over 27bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC38142.2141849488833283No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC33901.9680353898045317No Hit
ACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG24411.4171015889418472Illumina PCR Primer Index 6 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC20011.1616633672563033No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC19141.1111562643321162No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG17140.995047981747778Illumina PCR Primer Index 6 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG16540.9602154969724765Illumina PCR Primer Index 6 (96% over 27bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC16020.9300273435005486No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT14650.850493169930277No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT14080.8174023093937406No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13460.7814087417925959No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC12630.7332238045200955No Hit
CATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG12300.7140659378936797Illumina PCR Primer Index 6 (96% over 27bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT12070.7007134853964808No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTA11060.6420788026913901No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT11030.6403371784526249No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10120.587507909876751No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT9720.5642862533598835No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATA9230.5358397241267205No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT9030.5242288958682868No Hit
CACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG8910.5172623989132266RNA PCR Primer, Index 37 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG8680.5039099464160276No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC8350.4847520797896118No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT7750.44991959501431034No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG7570.43946984958171986No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT7490.4348255182783464No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC6990.4057984476322618No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT6890.39999303350304494No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA6760.39244599513506295No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC6750.39186545372214127No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA6450.37444921133449055No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT6130.3558718861209964No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG5730.3326502296041288No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA5300.3076869488484961No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA5260.3053647831968093No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT4630.26879067418274283No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4510.26182417722768253No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA4490.26066309440183916No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC4490.26066309440183916No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATG4480.26008255298891747No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT4350.2525355146209355No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG4210.24440793484003182No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA4180.24266631060126673No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT4110.23860252071081492No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG4070.23628035505912814No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT3650.21189761571641713No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG3640.21131707430349544No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT3620.21015599147765204No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC3500.20318949452259177No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA3430.1991257046321399No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC3300.19157866626415795No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG3290.19099812485123627No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC3270.18983704202539287No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC3260.1892565006124712No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCT3160.18345108648325428No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT3110.18054837941864582No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT3080.17880675517988076No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG3070.17822621376695907No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT2990.17358188246358552No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2980.17300134105066386No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC2910.16893755116021203No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT2860.16603484409560357No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2840.16487376126976017No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT2820.1637126784439168No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATG2820.1637126784439168No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2690.15616564007593484No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT2690.15616564007593484No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT2540.14745751888210945No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGA2540.14745751888210945Illumina PCR Primer Index 6 (96% over 27bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2530.14687697746918776Illumina PCR Primer Index 6 (96% over 27bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACC2520.14629643605626608No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCCCACG2490.144554811817501No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAT2370.13758831486244072No Hit
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGT2280.13236344214614548No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA2280.13236344214614548No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC2250.13062181790738042No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAT2190.12713856942985027No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACG2120.12307477953939845No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA1900.11030286845512124No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC1860.1079807028034345No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTC1850.10740016139051278Illumina PCR Primer Index 6 (95% over 21bp)
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCC1820.10565853715174772No Hit
CATCCATAGCGTCCTCAGCTGTCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT1810.10507799573882602No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG1790.10391691291298266Illumina PCR Primer Index 6 (96% over 27bp)
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA1760.10217528867421757No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC1760.10217528867421757No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1760.10217528867421757No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC1740.10101420584837419No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)