FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002293872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319683
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGC159724.996199359990991No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGC137764.309268869473822No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCAC85682.6801550285751823No Hit
CTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG56801.7767601029770115TruSeq Adapter, Index 1 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC47951.4999233615800653No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA45941.4370485762458436No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG34411.0763787877366016RNA PCR Primer, Index 1 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG32241.0084990443658248No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG30860.9653312813005385No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA30720.9609519430185528No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATG29130.9112151725302877No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG27980.8752420366425491No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG27730.8674217897104319No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA27280.8533453452326211No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA25280.790783369775684No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG22390.70038131524041No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG21370.6684747077573722No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA20450.6396961990471811No Hit
ACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG20130.6296862829740713RNA PCR Primer, Index 1 (96% over 28bp)
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGA18910.5915234779453395TruSeq Adapter, Index 1 (96% over 28bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA18390.575257364326536No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT17430.5452276161072062No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA15330.4795375418774223No Hit
ATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG14860.46483547764504207TruSeq Adapter, Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA13960.43668258868942045No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT13930.4357441590575664No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13280.41541151703406193No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG13230.4138474676476384No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC12800.400396642924397No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA12780.3997710231698276No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCT12500.3910123466058564No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT12350.38632019844658616No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC11900.3722437539687753No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT10910.34127557611759146No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATG10850.3393987168538834No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC10820.33846028722202937No Hit
CATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG10520.32907599090348877TruSeq Adapter, Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA10440.3265735118852113No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT10380.32469665262150316No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCC10370.3243838427442185No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC10160.3178148353212401No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC9570.2993590525614437No Hit
CACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG9540.2984206229295896TruSeq Adapter, Index 1 (96% over 28bp)
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC9470.2962309537885968No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG9430.29497971427945807No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG9350.2924772352611806No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC8850.27683674139694636No Hit
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCC8220.2571297191280112No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA7830.24493013391390844No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT7820.24461732403662376No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG7810.24430451415933913No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC7680.2402379857546382No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG7590.23742269685907602No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT7470.2336689783316598TruSeq Adapter, Index 1 (95% over 21bp)
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA7350.2299152598042436No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG7240.22647435115411202No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC7180.22459749189040393No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCT7140.2233462523812652No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA6740.21083385728987777No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC6710.20989542765802371No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCT6620.20708013876246156TruSeq Adapter, Index 1 (95% over 23bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG6340.19832146219849037TruSeq Adapter, Index 1 (96% over 28bp)
GTCCAGGGTGGGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCT6280.19644460293478228TruSeq Adapter, Index 1 (95% over 23bp)
GTGTATAAGAGACAGCGTGCCCATGCCCACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA6140.19206526465279666No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT6070.18987559551180389No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC6060.1895627856345192No Hit
GACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG6010.18799873624809577No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCA5970.18674749673895702No Hit
GTCCAGGGTGGGCATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC5840.18268096833425612No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC5770.18049129919326334No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC5700.17830163005227054No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA5650.1767375806658471No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA5450.1704813831201534No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA5420.16954295348829934No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC5230.16359956581989032No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT5190.16234832631075158No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCT5160.16140989667889752No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA5140.16078427692432817No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGT5130.1604714670470435No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCT4980.15577931888777322No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATATCGTATGC4920.1539024596240651No Hit
GTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTC4910.1535896497467804TruSeq Adapter, Index 1 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC4760.1488975015875101No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA4760.1488975015875101No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG4690.14670783244651733No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA4610.14420535342823984No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC4600.14389254355095515No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG4570.1429541139191011No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA4400.13763634600526145No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC4380.13701072625069208No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4360.13638510649612273No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC4320.13513386698698399No Hit
GTCCAGGGTGGGCATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGT4280.13388262747784524No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACA4190.1310673385822831No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATATCGTATGC4020.12574957066844342No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC3990.12481114103658937No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA3970.12418552128202001No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCC3970.12418552128202001No Hit
ATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA3900.1219958521410272No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC3890.12168304226374252No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG3820.11949337312274971No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTG3750.11730370398175692TruSeq Adapter, Index 1 (96% over 28bp)
GGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTG3710.1160524644726182TruSeq Adapter, Index 1 (95% over 24bp)
GTCCAGGGTGGACATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGT3620.11323717557705601No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG3610.11292436569977135No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC3610.11292436569977135No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3560.1113603163133479No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA3470.10854502741778574No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC3360.1051041187676542No Hit
GATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGA3340.10447849901308484No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC3320.10385287925851548No Hit
GTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT3300.1032272595039461No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT3270.10228882987209204No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAG3230.10103759036295329No Hit
AGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC3220.10072478048566863No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC3210.10041197060838393No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTC3200.10009916073109924TruSeq Adapter, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)