FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002293874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503359
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC122210.8129129502667028No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC78740.5237604590786366No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGC64890.4316334288749394No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC61790.4110129383600324No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG52580.34975012621735724TruSeq Adapter, Index 7 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC46810.31136940677509495No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC35860.23853251285953656No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGAC24050.15997509576887492No Hit
ACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG22170.1474697660372539RNA PCR Primer, Index 7 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG21920.14580682325379368RNA PCR Primer, Index 7 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCA21020.1398202292333368No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG17130.1139448395226955TruSeq Adapter, Index 7 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)