FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002293876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012797
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC359081.1918493014962508No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC290280.9634900725140126No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGC230770.7659659777940564No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC210830.6997816314872858No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG183610.6094336923463479Illumina PCR Primer Index 7 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC154610.5131776219904627No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC119690.3972720365826174No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG83090.2757902374438105TruSeq Adapter, Index 18 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG68450.22719751778828776TruSeq Adapter, Index 18 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG61040.20260243222493918Illumina PCR Primer Index 7 (96% over 27bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCT59780.19842027192671793No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCA51220.1700081352975325No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG40280.13369636254948475No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTC39340.13057633820001813No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC39110.12981292798685076No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT37990.12609545216620965No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAT37340.12393798852030191No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAT36520.12121626515161825No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTC34680.11510898344627932No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC33830.11228768483239993No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCC33830.11228768483239993No Hit
CATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG32630.1083046750245702Illumina PCR Primer Index 7 (96% over 27bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTC30700.10189866758364403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)