FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002293899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330339
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC139311.0471766970674392No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58310.43830933318500026RNA PCR Primer, Index 39 (96% over 30bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC47590.35772836848352185RNA PCR Primer, Index 39 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC47290.35547330417284617RNA PCR Primer, Index 39 (95% over 21bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC42350.31833991185705296RNA PCR Primer, Index 39 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC36320.2730131192124714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG27990.2103975001860428RNA PCR Primer, Index 39 (96% over 30bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC23950.1800293008022767RNA PCR Primer, Index 39 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG22360.1680774599556955RNA PCR Primer, Index 39 (96% over 30bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA17970.13507835220947442No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA17670.13282328789879874No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG17270.12981653548456445RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)