FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002293924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882500
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGC130500.4527320034692107No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG128290.4450650477016479TruSeq Adapter, Index 6 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG56230.19507372072853427RNA PCR Primer, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG45570.15809193408499567RNA PCR Primer, Index 6 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGC45550.1580225498699046No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG35980.12482220294882913No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG33050.11465741543798785TruSeq Adapter, Index 6 (96% over 28bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT30770.10674761491760625No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC29710.1030702515177797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)