FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002293931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122553
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG153641.3686658892720434RNA PCR Primer, Index 47 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGC96420.8589349456105859No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG81050.7220149070912465RNA PCR Primer, Index 47 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG63090.5620224613002682RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG54400.4846096353579742RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG41060.36577337551100036RNA PCR Primer, Index 47 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG34790.3099185517298515No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGC34490.30724607212309796No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG31150.2774924658345753TruSeq Adapter, Index 9 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC30000.26724796067535345No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC29880.26617896883265196No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG29580.2635064892258984No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC26410.23526728804786945No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGC24250.21602543487924403No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT21350.19019146534729317No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG20570.18324301836973397No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGC19760.17602732343149943No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC19700.17549282751014875No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA18990.1691679591074987No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA18270.16275400805129023No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC17610.15687455291643246No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG16540.14734270898567817No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16060.14306674161487254No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCC14680.13077333542380626No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC14680.13077333542380626No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGT14400.12827902112416964No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC14310.12747727724214358No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG13300.11847992923274002No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG12960.11545111901175267RNA PCR Primer, Index 47 (96% over 29bp)
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAG12870.11464937512972662No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA12450.11090790368027167No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA12060.10743368019149208RNA PCR Primer, Index 47 (96% over 29bp)
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAG11630.10360312608847869No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG11560.10297954751356951No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT11510.10253413424577726No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC11430.10182147301730966No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT11430.10182147301730966RNA PCR Primer, Index 47 (95% over 21bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA11280.10048523321393289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)