FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002293939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272744
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG172180.7575864241639182TruSeq Adapter, Index 11 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC139270.6127834899135143No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG69390.3053137528907787RNA PCR Primer, Index 11 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG65980.290309863319406RNA PCR Primer, Index 11 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGC54290.23887424188557974No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG46800.2059184844399545TruSeq Adapter, Index 11 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC40060.17626270270650807TruSeq Adapter, Index 11 (95% over 21bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC31780.1398309708440546TruSeq Adapter, Index 11 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC30690.13503500614235478TruSeq Adapter, Index 11 (95% over 21bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG28160.12390308807327177TruSeq Adapter, Index 11 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG27410.12060311236109303No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG25020.11008718975828338TruSeq Adapter, Index 11 (96% over 30bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG24390.10731521016005321No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAG23530.10353123801008825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)