FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002293955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5580
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC300.5376344086021506No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.4121863799283154No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA220.39426523297491045No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC210.3763440860215054No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG200.35842293906810035No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC200.35842293906810035No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC200.35842293906810035No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA200.35842293906810035No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG190.34050179211469533RNA PCR Primer, Index 41 (96% over 29bp)
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC180.3225806451612903No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.2867383512544803No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT160.2867383512544803No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG150.2688172043010753No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG150.2688172043010753No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC150.2688172043010753No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC140.25089605734767023No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.25089605734767023No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.25089605734767023No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGA140.25089605734767023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG140.25089605734767023RNA PCR Primer, Index 41 (96% over 29bp)
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC140.25089605734767023No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT130.23297491039426527No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC130.23297491039426527No Hit
GCCAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTA130.23297491039426527No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC130.23297491039426527No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC130.23297491039426527No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT130.23297491039426527No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC130.23297491039426527No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA120.21505376344086022No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC120.21505376344086022No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT120.21505376344086022No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCAC120.21505376344086022No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGA120.21505376344086022No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG120.21505376344086022No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAG120.21505376344086022No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC110.19713261648745523No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT110.19713261648745523No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA110.19713261648745523No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC110.19713261648745523No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC110.19713261648745523No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG110.19713261648745523No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC110.19713261648745523No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACG110.19713261648745523No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA110.19713261648745523No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG100.17921146953405018No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGA100.17921146953405018No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC100.17921146953405018No Hit
CCTGGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGGA100.17921146953405018No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA100.17921146953405018No Hit
GAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC100.17921146953405018No Hit
CTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCTTT100.17921146953405018No Hit
AGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGAC100.17921146953405018No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAG100.17921146953405018No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG90.16129032258064516No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCAC90.16129032258064516No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCC90.16129032258064516No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGA90.16129032258064516No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG90.16129032258064516No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC90.16129032258064516No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT80.14336917562724014No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGA80.14336917562724014No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC80.14336917562724014No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.14336917562724014No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT80.14336917562724014No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCA80.14336917562724014No Hit
CTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATCAGATCCTACGGTACTTATTGCCAAGAA80.14336917562724014No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC80.14336917562724014No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG80.14336917562724014No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC80.14336917562724014No Hit
GCTCCAGTATTCCAGGGTTCATCAGATCCTACGGTACTTATTGCCAAGAAAGCACGAGCATCAGCCGTGCCTCCA80.14336917562724014No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC80.14336917562724014No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC80.14336917562724014No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA70.12544802867383512No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC70.12544802867383512No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA70.12544802867383512No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG70.12544802867383512RNA PCR Primer, Index 41 (96% over 29bp)
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA70.12544802867383512No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATG70.12544802867383512No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATC70.12544802867383512No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG70.12544802867383512No Hit
AAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCT70.12544802867383512No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGAC70.12544802867383512No Hit
AAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC70.12544802867383512No Hit
CTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA70.12544802867383512No Hit
AGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCC70.12544802867383512No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT70.12544802867383512No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCC70.12544802867383512No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC70.12544802867383512No Hit
GTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAG70.12544802867383512No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA70.12544802867383512No Hit
GTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT60.10752688172043011No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG60.10752688172043011No Hit
GCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGT60.10752688172043011No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCC60.10752688172043011No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT60.10752688172043011No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAA60.10752688172043011No Hit
CTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG60.10752688172043011No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCG60.10752688172043011No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGA60.10752688172043011No Hit
TCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC60.10752688172043011No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACAT60.10752688172043011No Hit
GATCTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTG60.10752688172043011No Hit
GATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCT60.10752688172043011No Hit
GTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCT60.10752688172043011No Hit
CATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA60.10752688172043011No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA60.10752688172043011No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCC60.10752688172043011No Hit
GAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCTTTCTT60.10752688172043011No Hit
CCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCT60.10752688172043011No Hit
GAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCA60.10752688172043011No Hit
CTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATG60.10752688172043011No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCAC60.10752688172043011No Hit
CCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATCAGATCCTACGGTA60.10752688172043011No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG60.10752688172043011No Hit
CATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAA60.10752688172043011RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)