FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002293971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873180
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGC95510.5098815917317077No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG61190.32666374827832884Illumina PCR Primer Index 10 (96% over 29bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGC42370.22619289123309025No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC34650.1849795534865843No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC28660.15300184712627724No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC27430.1464354733661474No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG24890.13287564462571672Illumina PCR Primer Index 10 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG22390.11952935649537151Illumina PCR Primer Index 10 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG21200.1131765233453272No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA20090.10725077141545394No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA19180.10239272253600828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)