FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002293994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002293994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391670
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG170061.221985097041684TruSeq Adapter, Index 10 (96% over 28bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA53480.38428650470298276Clontech Universal Primer Mix Short (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG50360.36186739672479823TruSeq Adapter, Index 10 (96% over 28bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT46690.3354962024043056No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG45580.32752017360437463No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT42210.30330466274332274Clontech Universal Primer Mix Short (95% over 21bp)
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA37530.26967600077604603No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG35020.2516401158320579No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTCCGAGCCCA34280.24632276329877054Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT33950.24395151149338565No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA31690.22771202943226485No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTCCGAGCCCACG30820.22146054739988646Clontech Universal Primer Mix Short (95% over 22bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC30340.21801145386478116No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT29260.21025099341079423Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC27380.1967420437316318Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAG27160.1951612091947085No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCAC23810.17108941056428606No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCAC23370.16792774149043954No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA22640.1626822450724669Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTCCGAGCCC21210.15240682058246569Clontech Universal Primer Mix Short (95% over 21bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAG19500.140119424863653No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC19020.13667033132854772No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG18990.13645476298260364RNA PCR Primer, Index 10 (96% over 28bp)
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG18750.134730216215051No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC18240.1310655543340016No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG18210.13084998598805753No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGC18050.12970028814302242No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC17640.12675418741511996No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG17570.1262511946079171No Hit
TCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC17150.12323323776469997Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA16770.1205027053827416No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG16500.11856259026924486No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACA16250.1167661873863775Clontech Universal Primer Mix Short (95% over 21bp)
GCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA15950.1146105039269367Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTCCGAGCCCAC15900.11425122335036322Clontech Universal Primer Mix Short (95% over 22bp)
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC15790.1134608060819016No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTC14600.10490992835945304No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA14570.10469436001350894No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTCCGAGCCCA14460.10390394274504731No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA14280.10261053266938283No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC14220.10217939597749466No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA13940.10016742474868323No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)