FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002294020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760689
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA31120.40910280022453327Clontech Universal Primer Mix Short (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG31070.4084455013809849RNA PCR Primer, Index 33 (96% over 29bp)
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT25160.33075277807356224Clontech Universal Primer Mix Short (95% over 21bp)
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG22750.2990709738145287No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTCCGAGCCCA21830.2869766750932379Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT19100.2510881582354944Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTCCGAGCCCACG16600.21822321605807368Clontech Universal Primer Mix Short (95% over 22bp)
ATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC15920.20928395178581521No Hit
TCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC13810.18154594058807214Clontech Universal Primer Mix Short (95% over 21bp)
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC13330.17523587169000734Clontech Universal Primer Mix Short (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG12470.16393033158097461RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11950.1570944236080711No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA11900.1564371247645227Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG11170.14684056164871584No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA11130.14631472257387712No Hit
GTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC10810.14210800997516726No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10390.13658669968936057No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCAC10350.13606086061452183No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGACTGTCTCTTATACACATCTCCGAGCCCAC10330.13579794107710247Clontech Universal Primer Mix Short (95% over 21bp)
GCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT9610.12633283773000528No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACA9610.12633283773000528Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTCCGAGCCC9230.12133736651903734Clontech Universal Primer Mix Short (95% over 21bp)
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC9090.11949692975710179No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTCCGAGCC8700.11436999877742415No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA8340.10963744710387557No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACCTGTCTCTTATACACATCTCC8310.10924306779774651No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG8130.10687679196097223No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCG7960.10464197589290761No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT7870.10345883797452046No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCCTGTCTCTTATACACAT7820.10280153913097205No Hit
GCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA7790.10240715982484301Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)