FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTCTGTCT12.941176470588235No Hit
GGGCAAAACCTGTACTAGTAACCACATGTCCAGACCCCTCCTATGCTCCCACCCAGGGTCCCTTGAGCTGCTTCC12.941176470588235No Hit
CAGAACATCATTTTCACCATCCTGCCGTGGCTCTTTGTGCTGACGGTGGTCGTGGTGGTGCACGAGCTCGGCCAT12.941176470588235No Hit
TTACATGGGACCAATCAATACGAGAAAATAAATCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.941176470588235No Hit
CTTTGAGAGGCTGAGGCAGGCGGATCACGAGGTCAGAAGTTCGAGACCAGTCTGGCCAACATAGTGAAACCCTGT12.941176470588235No Hit
GTGTTGATTTGTCTGTTGCTTTTTGGATTCTCCAAGATCCAGGAAATGCTCATGAGCATGATTCTTTGAGACAGT12.941176470588235No Hit
CACCAGCAGCCGCCGGCCCTGAGCGACCAGGTCGGGCAGATGCTCGCGCAGCTGTCTCTTATACACATCTGACGC12.941176470588235No Hit
ATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT12.941176470588235No Hit
CAATTGACGTTACCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGACTAGT12.941176470588235No Hit
GTATCAACGCAGAGTACATGGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCT12.941176470588235No Hit
CAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGTTCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAC12.941176470588235No Hit
GATGAAGCCCAGCCGGCCCTGGTTGATGCCCAGCAGCGGGATGCCGTAACGCGCCATTTCGCGGGCAAAGCCGAG12.941176470588235No Hit
GTTTGAGAGCAACGCCATTGCCTACTATGTGAGCAATGAGGAGCTGCGGGGAAGTACTCCCTGTCTCTTATACAC12.941176470588235No Hit
GAGTATACCTATTTGTGTCTGCTGCTCTCCTACCTTCCTCCTACTCTGTCAATGTGATAGTTTGAAGCTGCGTAT12.941176470588235No Hit
GTGGTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCCTGTCTCTTATACACATCTGACGCTGCC12.941176470588235No Hit
GGAGAAGGCCAGGAGAGGCGGGGATGGTGTCCCCCGGGGGCCCGTACTGCACCTGTCTCTTATACACATCTGACG12.941176470588235No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGG12.941176470588235No Hit
GCCCTGGACGCCGGCGCTGATGCGCCAGGCGGAGGACCGGGCCTACCGGCTGGGACAGAAGCGCGACGTGCTGGT12.941176470588235No Hit
GTGTGGATGTAGGTGTGCTTCTTCATGTCGGACTTCTGGTGGAAACGCTTGCCGCAGAACTGGCAGGGGTAGGGC12.941176470588235No Hit
GAGGAGATGTGTCTCATGGAGAAGCATCCGGGCTCAGGTGACCTTCCCTGAAGACTTCCTGTCTCTGAGCAGCTC12.941176470588235No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCCATGTACTCTGCGTTGATACCTGTCT12.941176470588235Clontech Universal Primer Mix Short (95% over 21bp)
GCCACTCACTGACCCCCAGGACCAGGATGGCAAAGGATGAAGAGGACCGGAACTGACCAGCCAGCTGTCCCTCTT12.941176470588235No Hit
GTGCTTCGCAGCTGTCTGGGCGAGAGGCACAGCGATAGGCTCCGTGCTGAGCACCGACAGCGGCAAATCGGCGCC12.941176470588235No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCC12.941176470588235No Hit
GCGTTGATACCACTGCTGTCTCTTATACACATCTGACGCTGCCGACGACTAGTCGAGTGTAGATCTCGGTGGTCG12.941176470588235Illumina Single End PCR Primer 1 (100% over 21bp)
GGGTTGTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATCTGTCTCTTATACA12.941176470588235Clontech Universal Primer Mix Short (95% over 21bp)
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGG12.941176470588235No Hit
GGCTAAGGACGATCAGCTGAAGGTTCATGGGTTTTAAGTGCTTGTGGCTCACTGAAGCTTAAGTGAGGATTTCCT12.941176470588235No Hit
CGTGTTAGTAGCTCACAGTTAGTCTCCAGCAGCCCTGCGCTCCAGCTGCAAACATCTCATTAGACTCTGTCTCTT12.941176470588235No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGAC12.941176470588235No Hit
GCCTGCACCCGGCCGTTGGCGCCGGGCTCGCGGCGCAGGCGCAGCGATTCCAGTGCCAGTGCGGGATCGCGGCGC12.941176470588235No Hit
GTGTCAAGTCGGGAGTCCTGAACCACAGAGCCTGCTGGTTCTTCTCCTGGCTGCCACTTGAAAAGAAAAATTAAC12.941176470588235No Hit
GGTACATCTCGCCTGACCAGCTGGCTGACCTGTACAAGTCCTTCATCAAGGACTACCCAGTGGCTGTCTCTTATA12.941176470588235No Hit
GCCCTGAGTGGCCACTGCCAAGCTGCGGGGAAGGGTCCTGAGCAGGGGCATCTGGGAGGCTCTGGCTGCCTTCTG12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)