FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT811.428571428571429No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCT57.142857142857142Illumina PCR Primer Index 7 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT34.285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAGCTCGTATGCCGTCTT22.857142857142857No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG22.857142857142857No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG22.857142857142857No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACCCATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT22.857142857142857No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCTGNTTGAAAAAAAAA11.4285714285714286RNA PCR Primer, Index 7 (96% over 26bp)
CAACCAAAAGAACAGCCTGTCTCTTAGACAAATCTCCGAGCCCACGAGCCCAGTCCTCAGCTAGTATGCCGTCTT11.4285714285714286No Hit
CAAACAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACCAATCCTCATCTCGTATGCCATCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGCGCCCACGAGACCAATCCTCATCTCGTATGCCTTCTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCGCCGAGCCCACGAGACCACTACGCAGCGCGGAGGCCGTCTTCT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACCCATCTCCTAGCCCACGATACCAATCCTCATCTCTTATGCCGTCTTCT11.4285714285714286Illumina PCR Primer Index 7 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTAATACACAGCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCACATCGTATGCCGTCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGACACCAATCCTCATCTCTTATGCCGTCTT11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCCTCATCTCTTATTCCTTCTT11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCAACAAAACCAATCCTCAGCTCGTATGCCGTCTT11.4285714285714286No Hit
CAAGCAAAATACCAGCCTGTCTCTTATAACAATCTCCTATCCCACTATACCACTCCTCATCTCGTATGCCGTCTT11.4285714285714286RNA PCR Primer, Index 18 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGACCAATCCTCAACTCGTATGCCGTCTT11.4285714285714286No Hit
CTTCTTGTTTTCGGGACATTGAGCAAATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATC11.4285714285714286No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCACATCGTATGCCGTCAT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACCAATCCTCAACTCGTATGCCGTCTTC11.4285714285714286No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA11.4285714285714286No Hit
CAAGCAATCGAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAGCTCGTATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 7 (95% over 21bp)
CAAGCAATCAAGTCCAGTCTCTTATACACATCTCCGCTCCCACGAGACCAATCCTCATCTCTTATGCCGTCTTCT11.4285714285714286Illumina PCR Primer Index 7 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAACCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 18 (95% over 24bp)
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCAAGCCCACGAGACCACTCCTCATCTCTTATACCTTCTT11.4285714285714286No Hit
CAACGAAAAGAACAGCCTGTCTCTGATACACATCTCCGATCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT11.4285714285714286No Hit
CCCCCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATGCTCAGCTCGTATGCCGTCTT11.4285714285714286No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGTACCGCATTGACTACCTGA11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCTTCTT11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAACTCGTATGCCGTCTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACCAATCCTCATCTCTTATGCCGTCTTCT11.4285714285714286RNA PCR Primer, Index 7 (95% over 21bp)
CCTTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.4285714285714286No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACAGTGTAGAGAAGCGTGAAGATGCCGAA11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACCAATCCTCAGCTNGTATGCCGTCTT11.4285714285714286No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC11.4285714285714286No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTT11.4285714285714286No Hit
AAAGTCTCTGACCACTCTGTCTCTTATACACATCTCCGAGCCCACTAGCCCAGTCCTCATCTCGTATGCCTTCTT11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATATCGTATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 7 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGGAGACCAATCATCATCTCGTATGCCGTCTA11.4285714285714286No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGGAGCCCACTAGACCAATCCTCAGCTCGTATGCCGTCTT11.4285714285714286No Hit
CCAGCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTC11.4285714285714286TruSeq Adapter, Index 18 (95% over 23bp)
CAAGCAATCAAGACCATTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCAACTCGTATGCCGTCTTCT11.4285714285714286TruSeq Adapter, Index 7 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGATCCCACGAGACCAATCCTCAACTCGTATGCCGTCTTCT11.4285714285714286Illumina PCR Primer Index 7 (95% over 21bp)
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCCCGAGACCCATCCTCATCTCGTATGC11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT11.4285714285714286No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCACTGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG11.4285714285714286No Hit
CAAGCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACCAATCCTCAGCTCNTATGCCGTCTTA11.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGCGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCT11.4285714285714286Illumina PCR Primer Index 7 (95% over 22bp)
GAAGCAGTGGATCTCCGAGCCCACGAGACAGGCCGAAATCTCGTCTGCCGTCTTCTGCTTGAAAAAAAACAACAT11.4285714285714286RNA PCR Primer, Index 19 (96% over 26bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGACCAATCCTCATCTCGTATGCAGTCTT11.4285714285714286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)