FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT1758.333333333333332No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG1135.3809523809523805No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG994.714285714285714No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT924.380952380952381No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA884.190476190476191No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG864.095238095238096No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT854.0476190476190474No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG572.7142857142857144No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC552.619047619047619No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT512.4285714285714284No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA401.9047619047619049No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT401.9047619047619049No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA391.8571428571428572No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT371.7619047619047619No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC361.7142857142857144No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA341.6190476190476188No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT331.5714285714285716No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA311.4761904761904763No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA281.3333333333333335No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC261.2380952380952381No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC150.7142857142857143No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA130.6190476190476191No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT130.6190476190476191No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC110.5238095238095238No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG90.4285714285714286No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGNGAAGATGCCGAT90.4285714285714286No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG90.4285714285714286No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT70.33333333333333337No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA70.33333333333333337No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGGCTCTT70.33333333333333337No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA70.33333333333333337No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT60.2857142857142857No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATT60.2857142857142857No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGNGCCATTCAGGTA60.2857142857142857No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCGCTTGGCCTTGTCGCGGCTCTTG60.2857142857142857No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGNCCCCCTCAGTGA60.2857142857142857No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA60.2857142857142857No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCACGC50.2380952380952381No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGTGGCCCCCTCAGTGA50.2380952380952381No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCAC50.2380952380952381No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC40.19047619047619047No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCT40.19047619047619047No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCACTTTCAGCTCCATTGGGATCATG40.19047619047619047No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT40.19047619047619047No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC40.19047619047619047No Hit
ACCTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT40.19047619047619047No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAACAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA40.19047619047619047No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCACTG30.14285714285714285No Hit
ACCTCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA30.14285714285714285No Hit
ACATCAACCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA30.14285714285714285No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG30.14285714285714285No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCGTGTGCGGGGGGCCCCCTCAGTGA30.14285714285714285No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG30.14285714285714285No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCACTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT30.14285714285714285No Hit
GCCCAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG30.14285714285714285No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCNACAACTCCTGTT30.14285714285714285No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTTCCCCTGCGCACATG30.14285714285714285No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGC30.14285714285714285No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCGCTTGGCCTTNTCGCGGCTCTTG30.14285714285714285No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGCAGGGGCCCTG30.14285714285714285No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCTCATTGACTACCTGA30.14285714285714285No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCTCATTGACTACCTGA30.14285714285714285No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCTTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT30.14285714285714285No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCACACACTCGGAAGAAGTAGG30.14285714285714285No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGNCCCCCTCAGTGA30.14285714285714285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)