FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length75
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG211.11111111111111No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT211.11111111111111No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA15.555555555555555No Hit
TCTCGGGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC15.555555555555555No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT15.555555555555555No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG15.555555555555555No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT15.555555555555555No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCTGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC15.555555555555555No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACGCTCATGAATCTCGGATGCCGTCTTCTGCTGGAAAAAAATACCACG15.555555555555555Illumina PCR Primer Index 8 (96% over 25bp)
CAAGCAATCAAGGCCAGTCTACCCTGCAACCCTTGCCGCCTTCCCGTAAGCAGCACTCTGCACAGCATCATCCAT15.555555555555555No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGTCTTCTGCGTGAAAAAAAAAGGCAA15.555555555555555Illumina DpnII expression Adapter 2 (95% over 21bp)
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA15.555555555555555No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC15.555555555555555No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACTGCAGCAATAAGCTGGAGCACCGCATTTACTATCTGA15.555555555555555No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCTGGAGGCTGCGCGGGGCCCGCTCCCC15.555555555555555No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)