FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT412.903225806451612Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT39.67741935483871Illumina Paired End PCR Primer 2 (95% over 21bp)
CAACCACAAGAACAGCCTGTCTCTTATAGACATCTCCGAGCCCACGAGACAACCCACGATCTCGTATGCCGTCTT13.225806451612903TruSeq Adapter, Index 1 (95% over 22bp)
CAACCAAAAGAACAGCCTTTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT13.225806451612903Illumina Paired End PCR Primer 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTTTT13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGACCTCGTATGCCGTCCT13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTATT13.225806451612903No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG13.225806451612903No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG13.225806451612903No Hit
CCACCAAAAGGACAGCCTGTCTCTTAACACATCTCAGGGCCCACGAGACAACCAACGGACTCGTATGCCGGCTTC13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCTTCTT13.225806451612903No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA13.225806451612903No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACTATCTCGTATGCCTTCTT13.225806451612903No Hit
CAACCCACCGCACCGCCTTTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAACTCGTATGCCGTCTT13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCAGTCTT13.225806451612903No Hit
CAAGCACAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCACGATCTCGTCTGCCGTCTT13.225806451612903Illumina PCR Primer Index 1 (95% over 21bp)
CAAGCACTCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCT13.225806451612903Illumina Paired End PCR Primer 2 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCGCCGAGCCCACGAGACAACCAAAGAAATCGTATGCCGGCGA13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTC13.225806451612903Illumina Paired End PCR Primer 2 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCAAGACCACGAGACAACCAACGATCTAGTATGCCGTCTT13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACTATCTCGTATGCCGTCTT13.225806451612903RNA PCR Primer, Index 38 (95% over 21bp)
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT13.225806451612903Illumina Paired End PCR Primer 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAAACAACCAACGAGCTCGTATGCCGTCTT13.225806451612903No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCGCCGAGAGCACGGGACAACCAACGAGCTCGTAAGCCGTCTT13.225806451612903No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGACAACCAACGATCTCGTATGCCGTCTT13.225806451612903Illumina PCR Primer Index 1 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTT13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)