FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT2817.177914110429448No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT63.6809815950920246Illumina PCR Primer Index 6 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT63.6809815950920246No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTT53.067484662576687No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGTATGCCGTCTT21.2269938650306749No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGATACCCACGTATATCTCGTATGCCGTCTTCT21.2269938650306749RNA PCR Primer, Index 37 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACCCACGTATATCTCGTATGCCGTCTT21.2269938650306749No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCAGTCTT21.2269938650306749No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT21.2269938650306749No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCGT21.2269938650306749No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGACGTCTT21.2269938650306749No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTATATCTCGTATGCCGTCTTCT10.6134969325153374RNA PCR Primer, Index 37 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCCCGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGAACCACGTATATCTCGNATGACGTCTTCT10.6134969325153374No Hit
CAACCAAAAGAAAAGACAGTCTCTTATACACATCTCCGAGCCCACGAGACCCCCGCATATACCGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGTCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTC10.6134969325153374Illumina PCR Primer Index 6 (95% over 21bp)
GTCATTGTCACTGGTCATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAACAA10.6134969325153374RNA PCR Primer, Index 37 (96% over 28bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACAGCTCCGAGCCCACGAGACCCACGAATAGCACGTATGGAGTAAT10.6134969325153374No Hit
CCTTTGACAAGTATGCCTGTCAGCACCACACTCGTTTCCCCTTCTGCACTCAGCTCCCTTTCAACACCTCCTGTT10.6134969325153374No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTACCGGCCCC10.6134969325153374No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGACCACGAGACCCACGTATAACTAGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAAAAGAACAGCCTGTCTCTTCTACGCATCTCCGAGCCCACGAGACCCACGTATATCTCGGATGCCGTCTT10.6134969325153374No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT10.6134969325153374No Hit
CAACAAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGCGACACACGTATATATCGTATGCCGTCTTGT10.6134969325153374No Hit
CAACCAAAGGAACAGCCTGTCTCTTAGCCACATCTCCGAGACCACTAGACCCACGTATAACTCGGATGACGTCTG10.6134969325153374No Hit
CAACCAAAAGAACAGCCGGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAGATCGCGTATGCGGTCTT10.6134969325153374No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCCCGTATATCTCTTATACCGTCTT10.6134969325153374No Hit
CAACCAAAATAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGACCCACGTATAACTCGTATACCGTATT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTCTATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 16 (95% over 22bp)
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA10.6134969325153374No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTCTATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 16 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCCCGTATGCAGTCTT10.6134969325153374No Hit
CAATCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGATACCCACTTATATCTCATATGCCTTCTTCT10.6134969325153374No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTATT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTATCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 22bp)
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATCTCTCGTATGCCGCCTT10.6134969325153374No Hit
CAAGCAAAGGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATCTCGTCTGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 2 (95% over 21bp)
CCCCCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACTAGACCCACGTATAACTCGTATGCCTTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTG10.6134969325153374Illumina PCR Primer Index 6 (96% over 27bp)
CAAGCAAAAGAACAGCCTGTCCCTTATACACATCTCAGAGCCCACGAGACCCACGTACATCTTGTATGACGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACCTCTCCGAGCCCACGAGACCCACGTATATCTCTTATGCCTTCTTCT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACACACGTATATCTCGNATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACCCACGTATATCTAGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTTAT10.6134969325153374No Hit
CAAGCAAAAGAACAGCCTGTATCTTATACACATCTCCGAGCCCACGAGACACACGTATACCTNGTATGCCTTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATNTCGTAAGCCGTA10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGTATG10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGACCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374RNA PCR Primer, Index 37 (95% over 23bp)
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTNGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACTATACCCACGTATAACTCGTATGCCGTCTT10.6134969325153374No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACAAGACCCACGTATAGCTCGTATGCCGTGTT10.6134969325153374No Hit
CCCCCACAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACCCACATATATCTCGTATGCCGTCTT10.6134969325153374Illumina PCR Primer Index 2 (95% over 21bp)
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC10.6134969325153374No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTNCTTGAAAACAAA10.6134969325153374TruSeq Adapter, Index 6 (96% over 26bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACATATATCTCGTATGCCGTCTT10.6134969325153374Illumina PCR Primer Index 2 (95% over 21bp)
ATGCTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGC10.6134969325153374Illumina PCR Primer Index 6 (95% over 24bp)
CAAGCAATCAAGGCAAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAAACCCACGTATAGCTCTTATGCCGTCTTCT10.6134969325153374No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCTTATGCCGTCTT10.6134969325153374No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTAACTGTCGCTCTGTCCACTCTTAACGGCCCC10.6134969325153374No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACCCACGAATAACTCGTATGACGTATT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGACCCACGTATATATCGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACTATACCCACGTCTATCTCATATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTTCT10.6134969325153374Illumina Single End Adapter 1 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCACGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACGTCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCGGTCTTCT10.6134969325153374TruSeq Adapter, Index 25 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACCCACGTATATCTCGTATTCCGTCTTC10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCAAGCACACGATACCCACGTATATCTCGTATGACGTCTTAT10.6134969325153374No Hit
CAACCAAAAGAACAGCCGGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCACACGAGACCCACGTATATATCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACGTATAACTCGTATGCCGTATT10.6134969325153374No Hit
CACCCAAAAGAACAGCCTGTCTCTTCTACACATCTCCTAGCCCACGAGCCCCCCGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCAACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 22bp)
CAAGCAATAAGGCCAGTCTCTTATACATATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTG10.6134969325153374Illumina PCR Primer Index 6 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCTTATGCCGTCTTCT10.6134969325153374TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGTCCCACGTATCTCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 21bp)
AAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTATAGCTCGTATGACGGCT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATATCTCGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTATTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACACACGTATATCTCGTATGCCGTCTTCT10.6134969325153374RNA PCR Primer, Index 37 (95% over 23bp)
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATTCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTATTCT10.6134969325153374TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGNATGCCGTCTTCT10.6134969325153374TruSeq Adapter, Index 6 (95% over 21bp)
GAAGCAGTGGATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAACAAACAT10.6134969325153374TruSeq Adapter, Index 8 (96% over 29bp)
CAACCAAAAGAACAGCCTGTCGCTTATACACATATCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTG10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCGCTTATACACATCTCCGAGCCCACGAGACCCACGTAGAGCTCGTATGCCGGCGT10.6134969325153374No Hit
CAAGCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGACCCACGTGTAGCGCGTATGCCGTCGT10.6134969325153374No Hit
CAAGCAAACGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACCCACGTCTATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 16 (95% over 22bp)
CAATCAATCAATGCCAGTCTCTTATACACATCTCCGAGCCCACGATACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374RNA PCR Primer, Index 37 (95% over 23bp)
CAACCAAAACAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGGGACCCACGTATATCTCGTATGCCGTCTTC10.6134969325153374RNA PCR Primer, Index 37 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTAAAACTCGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTCTACACATCTCCGAGCCCCCGCGACCCACGTATAGCTCGTATGCCGTCTTCT10.6134969325153374Illumina Single End Adapter 1 (95% over 21bp)
CCTGACTCACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTTGAAAAAAAAAAGGA10.6134969325153374Illumina PCR Primer Index 6 (96% over 26bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCATCGTCT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACAAGACCCACGTGTATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 2 (95% over 23bp)
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATCGTATGACGTCTTCT10.6134969325153374No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGACCCCACGAGACCCACGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)
CCCGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT10.6134969325153374Illumina PCR Primer Index 6 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGTCTT10.6134969325153374No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACGTATATCTCGTATGCCGTCTT10.6134969325153374RNA PCR Primer, Index 37 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)