FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTT410.256410256410255Illumina PCR Primer Index 2 (95% over 21bp)
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG37.6923076923076925No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTT25.128205128205128Illumina PCR Primer Index 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCTTCTT12.564102564102564No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACAAGAGAGTATCTCGTATGCAGTATT12.564102564102564No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTTCACCACTTCTGCCCCTGCGCACATT12.564102564102564No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA12.564102564102564No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT12.564102564102564No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGTTGCGGGGTGCCCCCTCAGTGA12.564102564102564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCGCACGAGACAGGCGTGTATCTCGTATGCCGTCTT12.564102564102564Illumina PCR Primer Index 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACCCATCTCCGAGCCCCGAGACAAGCGTGTATCTCGTATGCCGTCTTC12.564102564102564RNA PCR Primer, Index 2 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT12.564102564102564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGAGTATATCGTATGCCGTATT12.564102564102564No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGTGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG12.564102564102564No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACAACCGTGTATCTCGTATGCCGTCTTCTGCCTGAAAAAAAAA12.564102564102564Illumina PCR Primer Index 2 (96% over 27bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT12.564102564102564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAAACATCTCCAAGCCCACTAGACAAGCGTGTATCTCGTATGCCGTCTT12.564102564102564Illumina PCR Primer Index 2 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGATCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCT12.564102564102564TruSeq Adapter, Index 2 (95% over 23bp)
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT12.564102564102564No Hit
GACAATTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA12.564102564102564No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTAGCTCGTATGACGTCTT12.564102564102564No Hit
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATCGTGTATCTCGTATGCCTTCTT12.564102564102564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCAGAGCCCACGAGACAAGCGTGTAGCTCGTATGCAGTATT12.564102564102564No Hit
CTTCAGTGGAACTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGTAAAAAAAAACCAGA12.564102564102564RNA PCR Primer, Index 47 (96% over 29bp)
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG12.564102564102564No Hit
CAAGCAAAAGAACAGCCTGTCTCTTACACACATCTCCGAGCCCACGAGACAAGCGTGTATTTCGTATGCCGTCTT12.564102564102564No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT12.564102564102564No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCCCGAGACAAGCGTGTATCTCGTATGCCGTC12.564102564102564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTT12.564102564102564Illumina PCR Primer Index 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATATCGTATGCCGTCTT12.564102564102564No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCAGTCTT12.564102564102564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGATACAAGCGTTTAACTCGTATGCCGTCTT12.564102564102564No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTGCTCAGAGGGACTCAGCACCGCCTGGCGGGTCGCGAAGGGGCCCTG12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)