FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT1117.1875TruSeq Adapter, Index 1 (95% over 21bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT812.5No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT46.25TruSeq Adapter, Index 1 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAGCTCGTATGCCGTCTT23.125No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGACTCTT23.125No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTNGTATGCCGTCTT11.5625No Hit
AAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGACCACTAGACGATGCACAAACTCGTAAGCCGAAAA11.5625No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT11.5625TruSeq Adapter, Index 1 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACGATGCACAAGCTAGTATGCCGTCTT11.5625No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAGCTCGTATGCCCTCTTCC11.5625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTGTGCCGTCTT11.5625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGGCGATGCACAAGCTCGTATGCCGTCTT11.5625No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTGGTCTGCGTGAAAAAAAAAAGCAA11.5625No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATATCGTATGCCGTCTTCTGCTTGAAAAAAAA11.5625TruSeq Adapter, Index 4 (96% over 26bp)
CAAACAATCAAGTCCAGTCTCTTATACACATCTCCGATCCCACGAGACGATACACAATCTCTTCTGCCGTCTTCT11.5625No Hit
CAACCAAAAGAACGGCCTGTCTTTATACACATCTCCGAGCCCACGCGACGATGCACAATCTCGTATGCCGTCTTC11.5625Illumina PCR Primer Index 1 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGATCCCACTAGACGATGCACAATCTCGTATGCCGTCTT11.5625Illumina PCR Primer Index 1 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATATCGTATGCCGTCTTCT11.5625TruSeq Adapter, Index 4 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACGATGCACAATCTCGTATGCCGTCTTC11.5625TruSeq Adapter, Index 1 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGNATGCAGTCTTCT11.5625No Hit
ACCTTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACTAGACTATGCACAATCTCGTATGCCGTCTT11.5625TruSeq Adapter, Index 1 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCAAGCCCACGAGACGATGCACAAACTCGTATGCCGGCTT11.5625No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAT11.5625RNA PCR Primer, Index 33 (96% over 29bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCATATGCCGTCTT11.5625No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACGATGCACACTCTCGTATGCAGTCTTCTG11.5625No Hit
CAACCAAAAGAACAGCCTATCTCTTATACACATCTCCGAGCCCACGAGACGCTGCACAATCTCATATGCCTTCTT11.5625No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCT11.5625TruSeq Adapter, Index 1 (95% over 23bp)
CCCCCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACCATCTCGTATGCCGTCTT11.5625TruSeq Adapter, Index 1 (95% over 21bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGGCTCTT11.5625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGGACGATGCACAATCTCGTATGCCGTCTTC11.5625Illumina PCR Primer Index 1 (95% over 22bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTTGCCTTGTCGCGTCTCTT11.5625No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACCTCTCTACGCCTCGTATGCCGTCTTCTGCTTGAAAGAAAAAATAGG11.5625TruSeq Adapter, Index 14 (96% over 25bp)
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTGGCGCTGCCTGGCCTTGTCGCGGCTCTT11.5625No Hit
ACCTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.5625TruSeq Adapter, Index 1 (96% over 28bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGCACAATCGCGTATGACGTCTT11.5625No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.5625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCAACGAGACGATGCACAATCTCGTATGCCGTCTT11.5625Illumina PCR Primer Index 1 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATAAACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT11.5625No Hit
CCCCCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGACGATGCACAAGCTCGTATGCCGTCTT11.5625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATACACAATCTCGTATGCCATCTT11.5625No Hit
CAACCAAAAGAACAGCCTGTCTCTTTACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTC11.5625TruSeq Adapter, Index 1 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT11.5625TruSeq Adapter, Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)