FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC790.883570070461917No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGA730.816463482831898No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA660.7381724639302092No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC530.5927748573985013No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA520.5815904261268314No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT470.5256682697684822No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC440.49211497595347276No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC440.49211497595347276No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT440.49211497595347276No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC430.48093054468180296No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC420.46974611341013306No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGA420.46974611341013306No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC390.4361928195951236No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA330.3690862319651046No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC320.35790180069343475No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA320.35790180069343475No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC310.3467173694217649No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG300.3355329381500951No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC300.3355329381500951No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC290.3243485068784252No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGA290.3243485068784252No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAG290.3243485068784252No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG280.3131640756067554No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCA280.3131640756067554No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG280.3131640756067554No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCG280.3131640756067554No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC270.30197964433508556No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAG270.30197964433508556No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC270.30197964433508556No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC260.2907952130634157No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC260.2907952130634157No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC250.2796107817917459No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA250.2796107817917459No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.2796107817917459No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC250.2796107817917459No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG240.2684263505200761No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.2684263505200761No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC240.2684263505200761No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCA240.2684263505200761No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC240.2684263505200761No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC230.2572419192484062No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.24605748797673638No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC220.24605748797673638No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCAC220.24605748797673638No Hit
GTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCG220.24605748797673638No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC220.24605748797673638No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACG220.24605748797673638No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG210.23487305670506653No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC200.2236886254333967No Hit
GTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC200.2236886254333967No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.2236886254333967No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCG200.2236886254333967No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGG190.2125041941617269No Hit
GGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG190.2125041941617269No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGA190.2125041941617269No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCG180.20131976289005704No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG180.20131976289005704No Hit
GTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCG180.20131976289005704No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAG180.20131976289005704No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC180.20131976289005704No Hit
GTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCG180.20131976289005704No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGA170.1901353316183872No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCA170.1901353316183872No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG170.1901353316183872No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC170.1901353316183872No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT170.1901353316183872No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT160.17895090034671737No Hit
GTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCG160.17895090034671737No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCC160.17895090034671737No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCAC150.16776646907504755No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC150.16776646907504755No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCAC150.16776646907504755No Hit
GTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT150.16776646907504755No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC150.16776646907504755No Hit
TTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC150.16776646907504755No Hit
AAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACT150.16776646907504755No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCA150.16776646907504755No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGA140.1565820378033777No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.1565820378033777No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG140.1565820378033777No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGG130.14539760653170786No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT130.14539760653170786No Hit
GAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCA130.14539760653170786No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG130.14539760653170786No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT130.14539760653170786No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG120.13421317526003804No Hit
CCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCAT120.13421317526003804No Hit
GTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCG120.13421317526003804No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC120.13421317526003804No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCG120.13421317526003804No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA120.13421317526003804No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA120.13421317526003804No Hit
TGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCT120.13421317526003804No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA120.13421317526003804No Hit
GTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGG120.13421317526003804No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG120.13421317526003804No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGA110.12302874398836819No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT110.12302874398836819No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC110.12302874398836819No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC110.12302874398836819No Hit
GTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCG110.12302874398836819No Hit
GTTCATCTCTTGAATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC110.12302874398836819No Hit
GTGTTCCTGTTCATCTCTTGAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT110.12302874398836819No Hit
CATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC100.11184431271669835No Hit
CATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT100.11184431271669835No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTGG100.11184431271669835No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCA100.11184431271669835No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACG100.11184431271669835No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGG100.11184431271669835No Hit
GTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGG90.10065988144502852No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC90.10065988144502852No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC90.10065988144502852No Hit
GTGTTCCTGTTCATTCAAGAGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTAT90.10065988144502852No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCC90.10065988144502852No Hit
GTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGGG90.10065988144502852No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC90.10065988144502852No Hit
TCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC90.10065988144502852No Hit
CATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTAT90.10065988144502852No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATC90.10065988144502852No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACG90.10065988144502852No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT90.10065988144502852No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAG90.10065988144502852No Hit
TTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC90.10065988144502852No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG90.10065988144502852Illumina PCR Primer Index 11 (96% over 29bp)
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG90.10065988144502852No Hit
CCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATCAGATCCTACGGTA90.10065988144502852No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCG90.10065988144502852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)