FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85288
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC50055.868351936966514No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC26093.0590469937154112No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG21172.4821780320795424RNA PCR Primer, Index 45 (96% over 30bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATC14901.7470218553606605No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCA14251.6708094925429136No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT12451.4597598724322296No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC11671.3683050370509333No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG10761.2616077291060876No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG10531.2346402776475003RNA PCR Primer, Index 45 (96% over 30bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC8581.0060031891942596No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT7790.9133758559234593No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATG7790.9133758559234593No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG7210.845370978332239No Hit
ACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG7010.8219210205421631RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGA6860.804333552199606RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA6430.7539161429509427No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC6230.7304661851608667No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT5880.6894287590282338No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATG5630.6601163117906388No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC5330.6249413751055248No Hit
CGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCG5070.5944564299784261No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT5010.5874214426414033No Hit
CATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG4600.5393490291717474RNA PCR Primer, Index 45 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG4420.5182440671606791RNA PCR Primer, Index 45 (96% over 30bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACG4320.5065190882656412No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA4220.49479410937060314No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC4110.48189663258606136No Hit
CACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG3690.43265172122690176RNA PCR Primer, Index 45 (100% over 29bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG3670.4303067254478942No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAT3660.42913422755839037No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA3610.4232717381108714No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATG3590.4209267423318638No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT3480.408029265547322RNA PCR Primer, Index 45 (95% over 23bp)
GTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACG3310.3880968014257575No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCT3150.36933683519369664RNA PCR Primer, Index 45 (96% over 25bp)
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCT3090.36230184785667385RNA PCR Primer, Index 45 (95% over 22bp)
GTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG3010.35292186474064347No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC2970.34823187318262827No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCA2940.3447143795141169No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC2910.3411968858456055No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2860.3353343963980865No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC2810.3294719069505675No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTG2630.3083669449394991No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC2600.3048494512709877RNA PCR Primer, Index 45 (95% over 21bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT2450.28726198292843075No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA2390.2802269955914079No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCT2330.27319200825438517No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTG2210.25912203358033953No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC2200.2579495356908358No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG2160.2532595441328206No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2060.24153456523778255RNA PCR Primer, Index 45 (96% over 30bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCA2040.239189569458775No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAG1950.22863708845324077No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTC1950.22863708845324077No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC1930.2262920926742332No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC1910.2239470968952256No Hit
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCT1880.22042960322671418RNA PCR Primer, Index 45 (95% over 22bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA1830.21456711377919518No Hit
GTCCAGGGTGGACATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGT1810.2122221180001876No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCG1800.21104962011068382No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA1750.20518713066316482No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTC1670.1958071475471344No Hit
GTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTC1640.192289653878623RNA PCR Primer, Index 45 (95% over 24bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC1620.18994465809961542No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT1590.18642716443110402No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAA1580.18525466654160022RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTG1460.17118469186755464RNA PCR Primer, Index 45 (96% over 30bp)
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC1420.16649470030953945No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCT1410.16532220242003565No Hit
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG1410.16532220242003565No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTC1370.16063221086202045No Hit
GGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTA1340.15711471719350906No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTG1300.15242472563549386No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTC1300.15242472563549386RNA PCR Primer, Index 45 (95% over 24bp)
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTG1290.15125222774599006No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCC1290.15125222774599006No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG1290.15125222774599006No Hit
GGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGC1290.15125222774599006RNA PCR Primer, Index 45 (96% over 27bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCA1280.15007972985648627No Hit
GACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCG1250.14656223618797487No Hit
GTCCAGGGTGGACATGGGCACGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTA1250.14656223618797487No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC1240.14538973829847104No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACG1230.14421724040896725No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCA1230.14421724040896725No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1200.14069974674045588No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG1200.14069974674045588No Hit
GATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCC1200.14069974674045588No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG1190.13952724885095208RNA PCR Primer, Index 45 (96% over 30bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC1190.13952724885095208No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC1170.13718225307194448No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC1160.13600975518244068No Hit
GTTTCCAGGTCAAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT1150.13483725729293686RNA PCR Primer, Index 45 (95% over 23bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATC1120.13131976362442546No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTC1120.13131976362442546No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC1110.1301472657349217No Hit
GCGTCAGATGTGTATAAGAGACAGGGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCC1090.1278022699559141No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGCTGTCTCTTATACACATCTCCGAGCCCACGA1060.12428477628740268No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTG1050.12311227839789887RNA PCR Primer, Index 45 (96% over 26bp)
GGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAA1010.11842228683988369RNA PCR Primer, Index 45 (96% over 30bp)
CGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCG1010.11842228683988369No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATGTCGTATGC1000.1172497889503799No Hit
GTCCAGGGTGGACATGGGCACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATG990.1160772910608761No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG980.1149047931713723RNA PCR Primer, Index 45 (96% over 30bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT970.1137322952818685No Hit
GTACTACCGACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTG950.11138729950286089RNA PCR Primer, Index 45 (96% over 26bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTC950.11138729950286089No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG950.11138729950286089RNA PCR Primer, Index 45 (96% over 30bp)
GGTCGGTAGTACCTGTACGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCG950.11138729950286089No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGC940.11021480161335709No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC940.11021480161335709No Hit
GTCCAGGGTGGGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCT930.10904230372385329RNA PCR Primer, Index 45 (96% over 25bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTC920.1078698058343495No Hit
GATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGC910.1066973079448457No Hit
GGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTG910.1066973079448457RNA PCR Primer, Index 45 (96% over 26bp)
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC900.10552481005534191No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCA890.10435231216583811No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC870.1020073163868305No Hit
ATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTC870.1020073163868305No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCAG860.1008348184973267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)