FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264502
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC97210.7687611407494809No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGC42870.3390267472886559No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39550.31277135188398275RNA PCR Primer, Index 45 (96% over 30bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC36400.28786035925605497RNA PCR Primer, Index 45 (95% over 21bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC31230.24697469833974164RNA PCR Primer, Index 45 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC28840.22807397694902815RNA PCR Primer, Index 45 (95% over 21bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTC23570.1863974908699235RNA PCR Primer, Index 45 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG16130.12756009875824634RNA PCR Primer, Index 45 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC15650.1237641379768478No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG14380.11372065840939755RNA PCR Primer, Index 45 (96% over 30bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGC13610.10763130465590406No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG12770.10098837328845664No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCAT12650.100039383093107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)