FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29004
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG9583.302992690663357TruSeq Adapter, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG5541.9100813680871602TruSeq Adapter, Index 25 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG3691.2722383119569713TruSeq Adapter, Index 25 (96% over 28bp)
CATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG3691.2722383119569713TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG3391.1688043028547785TruSeq Adapter, Index 6 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGC2370.8171286719073232No Hit
CACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG2340.8067852709971038RNA PCR Primer, Index 31 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC1230.4240794373189905No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC1210.4171838367121776No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC1110.3827058336781134No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC970.3344366294304234No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGC910.3137498276099848No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGA630.21721141911460487TruSeq Adapter, Index 6 (96% over 28bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG530.1827334160805406TruSeq Adapter, Index 6 (96% over 28bp)
TCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG420.1448076127430699TruSeq Adapter, Index 6 (96% over 28bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATG390.13446421183285062No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAT370.1275686112260378No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGA370.1275686112260378No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCG340.1172252103158185No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATA300.1034340091021928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)