FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106734
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4990.46751737965409335Illumina PCR Primer Index 2 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG2280.21361515543313284Illumina PCR Primer Index 2 (96% over 28bp)
CATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG1790.1677066351865385Illumina PCR Primer Index 2 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG1510.14147319504562744Illumina PCR Primer Index 2 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC1430.13397792643393858No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG1180.11055521202241085Illumina PCR Primer Index 2 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG1180.11055521202241085Illumina PCR Primer Index 2 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph