FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056538
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA50350.24482893095094765No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTC37890.18424167216944204No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG37100.1804002649112246No Hit
GTCGGTGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACAGATTGGGAACG32410.1575949484035792No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAACGATCGTTCCCAATCTGTCCCCCGGCCGGCACG31480.15307278542871564No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG31200.15171127399542336RNA PCR Primer, Index 27 (96% over 30bp)
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCG28170.13697777527086782No Hit
TTCCCAATCTGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAAGCAGCTCGAGCAGTCCACCGACAG28140.13683189904587223No Hit
GTACTTGTTTGCTATCGGTCTCTCGCCCATATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC27710.13474100648760198No Hit
GTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG26470.12871145585445054No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTC26050.12666918870451216No Hit
GATTGGGAACGATCGTTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAA25480.12389754042959576No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAACGATCGTTCCCAATCTG24690.12005613317137831No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGAC24530.11927812663806844No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC23650.11499909070486419No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT22660.11018517528000943No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA22570.1097475466050226No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTT21370.10391249760519863No Hit
AAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC21190.10303724025522505No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCA21150.10284273862189758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)