FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002294148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699351
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGC447181.2088066258108516No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG380381.02823441192793RNA PCR Primer, Index 41 (96% over 29bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGC169190.4573504920187352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG167120.45175491592984823RNA PCR Primer, Index 41 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC136650.36938911717217426No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG132840.35909001335639684RNA PCR Primer, Index 41 (96% over 29bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC125620.3395730764666559No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC115360.31183848193912933No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG104510.28250901306742726RNA PCR Primer, Index 41 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG73780.19944038832757421No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC71760.19397997108141402No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA71310.19276354149687336No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG66110.17870702185329265No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG59280.16024432393682025RNA PCR Primer, Index 41 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTA58690.15864944959264476No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG54290.14675547143269185No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG47850.12934701248948802No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG44960.1215348313798826TruSeq Adapter, Index 3 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACA39290.10620781861467052No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGA37840.10228821217559511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)