FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823755
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGC77540.425166757596278No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGC44100.24180879558931986No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGT40490.22201447014538683No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39820.21834073107407517TruSeq Adapter, Index 7 (96% over 28bp)
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCT38940.21351552154757628No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCA35240.1932277087657059No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAAC34780.1907054401495815No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGT34680.19015712088520662No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGT33170.181877499993146No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCAC33170.181877499993146No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGC31630.17343338332177294No Hit
GTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCT30700.1683340141630866No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATAC30080.16493443472396238No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCC29070.15939641015377612No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGC28990.15895775474227622No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGC28850.1581901077721514No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC28090.15402288136290235No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGA27360.15002015073296576No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT27180.149033176057091No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTC26020.14267267259034244No Hit
GTATCAACGCAGAGTACATGGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCAT25760.14124704250296777No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGA25300.13872477388684334No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCT25260.1385054461810934No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTA25140.13784746306384354No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACA25110.13768296728453108No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGC25070.13746363957878113No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAG24740.13565418600634405No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG24740.13565418600634405No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTC24650.13516069866840666No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCA24590.13483170710978173No Hit
ATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCACCAT24450.1340640601396569No Hit
GTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA24380.1336802366545945No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG23640.12962267409822043No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCT22940.1257844392475963No Hit
AGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG22650.12419431338090918No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCT22630.1240846495280342No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTG22420.12293317907284697No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCT22200.12172687669122224No Hit
GCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCTCAGG22200.12172687669122224No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACG22150.1214527170590348No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC21860.11986259119234767No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCA21860.11986259119234767No Hit
ACCTTACCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCACCATGGTGGGTTCTCTCTGAGTCTGT21840.1197529273394727No Hit
CACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGG21580.11832729725209802No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA21500.11788864184059812No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC21110.11575019670953608No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTT20940.1148180539600988No Hit
GACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAG20940.1148180539600988No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGC20790.11399557506353648No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC20780.113940743137099No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTT20640.11317309616697417No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTG20500.11240544919684935No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT20280.11119914681522462No Hit
GGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCCTTGACGT20130.11037666791866231No Hit
GGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGC19940.10933486131635005No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACT19900.1091155336106001No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGT19740.1082382227876003No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCC19720.10812855893472532No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC19710.10807372700828784No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA19680.10790923122897537No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG19550.10719641618528804No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTG19300.10582561802435085No Hit
GTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGG19080.10461931564272613No Hit
GTACATGGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACT19000.10418066023122623No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGG18790.10302918977603899No Hit
GGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCA18370.10072624886566452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)