EGAF00002294200 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00002294200
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20001
Sequences flagged as poor quality	0
Sequence length	75
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	1168	5.83970801459927	RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	974	4.8697565121743915	RNA PCR Primer, Index 39 (96% over 30bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	761	3.804809759512024	RNA PCR Primer, Index 39 (95% over 23bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG	675	3.3748312584370783	RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	638	3.1898405079746013	RNA PCR Primer, Index 39 (96% over 30bp)
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	336	1.6799160041997898	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG	272	1.3599320033998301	Illumina PCR Primer Index 11 (96% over 29bp)
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	267	1.3349332533373333	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG	135	0.6749662516874156	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG	130	0.6499675016249188	RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGG	124	0.6199690015499225	RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGGGG	116	0.5799710014499274	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG	110	0.5499725013749313	RNA PCR Primer, Index 39 (96% over 30bp)
GACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAA	109	0.544972751362432	RNA PCR Primer, Index 39 (96% over 30bp)
CGGAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	100	0.4999750012499375	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGG	88	0.439978001099945	RNA PCR Primer, Index 39 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG	87	0.43497825108744564	RNA PCR Primer, Index 39 (96% over 30bp)
GTGTATAAGAGACAGCAGAAGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	84	0.41997900104994745	No Hit
GGAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC	82	0.4099795010249488	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG	77	0.3849807509624519	RNA PCR Primer, Index 39 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTACTACCGACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC	75	0.3749812509374531	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGGGG	74	0.3699815009249538	RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG	67	0.3349832508374581	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	62	0.30998450077496126	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG	62	0.30998450077496126	RNA PCR Primer, Index 39 (96% over 30bp)
CTCACAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	58	0.2899855007249637	No Hit
GATGTGTATAAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	58	0.2899855007249637	No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	56	0.279986000699965	RNA PCR Primer, Index 39 (95% over 21bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGG	55	0.2749862506874656	RNA PCR Primer, Index 39 (96% over 30bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGTCTT	52	0.2599870006499675	TruSeq Adapter, Index 11 (95% over 21bp)
GTGTATAAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	50	0.24998750062496874	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG	49	0.24498775061246938	RNA PCR Primer, Index 39 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC	49	0.24498775061246938	No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCC	49	0.24498775061246938	No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCCGTCTT	48	0.23998800059997	TruSeq Adapter, Index 11 (95% over 21bp)
GTATAAGAGACAGCAGAAGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC	48	0.23998800059997	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGG	47	0.23498825058747064	Illumina PCR Primer Index 11 (96% over 29bp)
GAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA	46	0.22998850057497125	RNA PCR Primer, Index 39 (96% over 30bp)
CTCACAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC	44	0.2199890005499725	No Hit
CGTCAGATGTGTATAAGAGACAGCTCACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACAT	42	0.20998950052497373	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG	41	0.2049897505124744	RNA PCR Primer, Index 39 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	40	0.199990000499975	RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGGGGGGGG	39	0.19499025048747562	RNA PCR Primer, Index 39 (96% over 30bp)
GTACTACCGACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG	37	0.1849907504624769	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG	37	0.1849907504624769	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAGGGGG	36	0.17999100044997748	RNA PCR Primer, Index 39 (96% over 30bp)
GAGATGTGTATAAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	35	0.17499125043747812	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGG	34	0.16999150042497876	Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGG	34	0.16999150042497876	RNA PCR Primer, Index 39 (96% over 30bp)
GTACTACCGACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG	33	0.16499175041247938	RNA PCR Primer, Index 39 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGG	33	0.16499175041247938	RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGGGG	32	0.15999200039998	RNA PCR Primer, Index 39 (96% over 30bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA	31	0.15499225038748063	No Hit
GAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCC	31	0.15499225038748063	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGGG	31	0.15499225038748063	Illumina PCR Primer Index 11 (96% over 29bp)
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT	31	0.15499225038748063	No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC	29	0.14499275036248185	No Hit
CAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA	29	0.14499275036248185	RNA PCR Primer, Index 39 (96% over 30bp)
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCCGT	29	0.14499275036248185	No Hit
TGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	26	0.12999350032498375	RNA PCR Primer, Index 39 (95% over 22bp)
ATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	25	0.12499375031248437	RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAGGGGGGG	24	0.119994000299985	RNA PCR Primer, Index 39 (96% over 30bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG	24	0.119994000299985	Illumina PCR Primer Index 11 (96% over 29bp)
GTACTACCGACCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	24	0.119994000299985	No Hit
GTGTATAAGAGACAGGACGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	24	0.119994000299985	RNA PCR Primer, Index 39 (95% over 23bp)
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGTCTT	24	0.119994000299985	TruSeq Adapter, Index 11 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG	23	0.11499425028748562	Illumina PCR Primer Index 11 (96% over 29bp)
GATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGG	23	0.11499425028748562	No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC	23	0.11499425028748562	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG	22	0.10999450027498625	RNA PCR Primer, Index 39 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC	22	0.10999450027498625	No Hit
TCGGAGATGTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	22	0.10999450027498625	No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC	22	0.10999450027498625	No Hit
AGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA	22	0.10999450027498625	RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTGGGGGGGG	21	0.10499475026248686	RNA PCR Primer, Index 39 (96% over 30bp)
CTCACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA	21	0.10499475026248686	RNA PCR Primer, Index 39 (96% over 30bp)
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACAT	21	0.10499475026248686	No Hit

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content