FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002294209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778098
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG86731.1146410863413092RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG59420.7636570200668811No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA56020.7199607247416135No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC43930.5645818393055888No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA39990.5139455441345434No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA29400.37784443604790147No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG26020.334405177753959RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTCCGAGCC25620.32926443712745695No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC21120.2714311050793088No Hit
ACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA19340.24855480929137458No Hit
GCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT18400.23647406881909475No Hit
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGG17640.22670666162874087No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC17250.22169443951790135No Hit
ATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC17250.22169443951790135No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA16330.2098707360769466Clontech Universal Primer Mix Short (95% over 21bp)
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC16080.2066577731853828No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC15430.19830406966731698No Hit
CAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTC14910.1916211068528643No Hit
CTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC14820.19046444021190131No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG14140.18172518114684783RNA PCR Primer, Index 26 (100% over 29bp)
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT14010.18005444044323465Clontech Universal Primer Mix Short (95% over 21bp)
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT13470.1731144405974569No Hit
TAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA13260.17041555176854328No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTCCGAGC13160.16913036661191777No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG13030.1674596259083046No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG12430.15974851496855152No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTCCGAGCCCA12340.15859184832758857Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT12070.15512184840469967No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTG11490.14766777449627166No Hit
GGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCT11110.14278407090109474No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAG10830.13918555246254327No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTCCGAGCCCACG10790.13867147839989308Clontech Universal Primer Mix Short (95% over 22bp)
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTCTGTCTCTTATACACATCTCCGAGCCCA10670.13712925621194244No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTCCGAG10570.13584407105531693No Hit
GCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCT10450.13430184886736632No Hit
GCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA10440.13417333035170378No Hit
GAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTG9940.1277474045685762No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCC9570.12299221948906178No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCCTGTCTCTTATACACAT9480.12183555284809883No Hit
GGGCTCCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGC9450.12144999730111117No Hit
ACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC9380.12055036769147331No Hit
GAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTT9360.1202933306601482No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT9080.11669481222159678Clontech Universal Primer Mix Short (95% over 21bp)
CTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTG8660.1112970345637696No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC8490.10911221979750622Clontech Universal Primer Mix Short (95% over 21bp)
ATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGGG8460.10872666425051856No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTC8270.10628481245293009No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACCTGTCTCTTA8190.10525666432762969No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCCTGTCT8070.10371444213967905No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTACTGTCTCTTATACACATCTCCGAGCCCACGA8070.10371444213967905No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAGCCCA8040.1033288865926914No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC7960.10230073846739099No Hit
GTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGGGGCTGTCTCTTA7940.10204370143606588No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACA7820.10050147924811528Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)