FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length75
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT2921.96969696969697TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT53.787878787878788TruSeq Adapter, Index 6 (95% over 21bp)
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT43.0303030303030303TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCT32.272727272727273TruSeq Adapter, Index 6 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATATCGTATGCCGTCTT32.272727272727273RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTNGTATGCCGTCTT21.5151515151515151RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAACTCGTATGCCGTCTT21.5151515151515151RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAGCTCGTATGCCGTCTT21.5151515151515151RNA PCR Primer, Index 31 (95% over 21bp)
AAACTCCCTTACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAGCTCGTCTGCCGTCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGCTATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCATCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGATCCCACGAGACCAAGCGATATCGCGTAATCCGTATT10.7575757575757576No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATACCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAGAAGAACAGACTGGCTCTTATACACAGCTCCGAGCCCACGAGGCCACGCGGTATCTCGTATACCGTCTT10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATACCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTNATATGCCGTCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCCCACGCGATATCTCGTATGCCCTCTT10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTTACACATCTCCGAGCCCACGGACCAAGCGATAACTCGTATGCCGTCTTCT10.7575757575757576TruSeq Adapter, Index 25 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATACCTAGTATGCCGTCTT10.7575757575757576No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCTTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CTTCAGTGGAACATTCAGTGCACATGACTGGGTGACAGATTTCTTCCACATCTGTCTCTGCCTTCTCGGCAGCAT10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTNGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGCGATATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 23bp)
AAACGCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCGACGAGACCAAGCGATGGCGCGTATGCCGTCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACCAAGCGATATCTCGTATGCCTTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCGTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAAGTCGTATGCCGTCAT10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCCTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAACTCGTATGCCGTCAT10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGACCAAGCGATATCTCGTATGCCGACTT10.7575757575757576No Hit
CAAGCAAAATAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGCCCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 22bp)
CAAGCACTCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCGATATCTCGTATGCCGTCTTCT10.7575757575757576RNA PCR Primer, Index 31 (95% over 24bp)
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCCCGAGACCAAGCGATATCTCGT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAGCTCGTATGCCGGCTT10.7575757575757576No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGTTATCTCGTATGCCGTCTTCT10.7575757575757576RNA PCR Primer, Index 31 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAGATCGTATGCCGTCTT10.7575757575757576No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGATCCCACGAGACCACGCAATCACTCGTATGCCGTCTTCT10.7575757575757576No Hit
CAAGCAATCACGGCCATTCTCTTATACACATCTCCGATCCCACGATACCAAGCGATATCTCGTATGCCCTCTTCT10.7575757575757576RNA PCR Primer, Index 31 (95% over 23bp)
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTGATAAACATCTCCGAGCCCACGAGACCAAGCGGTAGCTCGTATGCCGTCTT10.7575757575757576No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGATCCCACGATACCAAGCGATAACTCGTATGCCGTCTTCT10.7575757575757576RNA PCR Primer, Index 31 (95% over 23bp)
CAACCAAAAGAACGGCCTGTCTCTTATACACATCTCCGAGCCAACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 22bp)
CAACCAAAAGAACAGCCTATCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATAAATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCT10.7575757575757576TruSeq Adapter, Index 6 (95% over 23bp)
CAACCAAAAGCACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CCTGTCTCTTATACACATCTCCGATCCCACGAGACCAAGCGATATATCGTATGCCGTCTTCTGCTTGAAAAAGAA10.7575757575757576TruSeq Adapter, Index 6 (96% over 26bp)
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCAAGCCCACGAGACTAAGCGATATCTCTTATGCCATTTT10.7575757575757576No Hit
CACCCCACCGCATCGCCTGTCTCTTCTACAAATCTCCGATCCCACGAGACCAAGCGATATCTCTTATGCCGTCTT10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACAAAAGACCAAGCGATATCTCGTATGCCGTCGT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGACTGTCTCTTATACACATCTCCGAGCCCACAAGACCACACGATATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (100% over 23bp)
CAAGCAACATCACAGCCTTTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAGCTCGTATGCCGTCTC10.7575757575757576No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGGAGACCAAGCGAGATAGCGTATGCCGTCTT10.7575757575757576No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGCTATCTCGTATGCCGTCTTCT10.7575757575757576RNA PCR Primer, Index 31 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCGCCGAGCCCACGAGACCAAGCGAGATCTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 17 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCAACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
AAACTCCCTAACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTNGTATGCCTTCTT10.7575757575757576No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCCCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CCCGCAAACGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCTAGCCCACGAGACCAAGCTATAGATCGTATGCCGTCTTCT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGCGATAGCTCGTAAGCCGGCAT10.7575757575757576No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTTTAATATGTGCTTTAGACTCTCCACT10.7575757575757576No Hit
ACACCAGCCAAAAGAACAGCCTGTCTCTTATAAACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCG10.7575757575757576No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGCGACCAAGCGATATCTCGTATTCCATCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATATNGTATGCCGTCAT10.7575757575757576No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGT10.7575757575757576No Hit
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAACTCGTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAGCGCGTATGCCGGCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAACCCAATCTATATCTCTTATGCCTTCTT10.7575757575757576No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGAGATATCTCGTATGCCGTCTTCT10.7575757575757576RNA PCR Primer, Index 17 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
ACCCTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCCCACGCGATAACTCGTATGCCGTCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCATCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGACCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCATCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAGCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAATAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACCAATCGATATCTCGTATGCCGTCTT10.7575757575757576TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTGATACAAATCTCCGAGCCCACGAGACAAAGCGAGAGCTAGTATGCCGTCTA10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGATACCAAGCTATCTCTCGTATGCCGTCTT10.7575757575757576No Hit
CAACCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATAACTCGTATGCCATCTT10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCTACGAGACCAAGCGATAGCACGTATGCAGTCTG10.7575757575757576No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGAGATAGCTCGTATGCCATCTT10.7575757575757576No Hit
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCGAGCCCACAAGACCAAAAAATAAATCATATTCCTTCTT10.7575757575757576No Hit
ACACTCCCTTACCAATCTGTCTCTTATACACCTCTCCGAGCCCACTAGACCAAGCGATATCTCTTATGCCTTCTT10.7575757575757576No Hit
CAACCAAAAGAACATCCTGTCTCTTATACACATCTCCGAGCCCCCGAGACCAATCGATATCTCGTATTCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCGCGTATGCCGTCTTCT10.7575757575757576RNA PCR Primer, Index 31 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATACCTCGTATGCAGTCTTCT10.7575757575757576No Hit
AAACTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACTAGACCAAGCGATATCTCTTATGCCGTCTT10.7575757575757576RNA PCR Primer, Index 31 (95% over 21bp)
GAAGCAGTGGATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGA10.7575757575757576RNA PCR Primer, Index 38 (96% over 26bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTAAGAGACCACGCGACCGAGCAATATCTAGGATGCAGTCTTCT10.7575757575757576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)