FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG1175.172413793103448No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG1165.128205128205128No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG1134.9955791335101685No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT974.288240495137047No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC954.199823165340407No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG773.404067197170645No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA773.404067197170645No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT733.227232537577365No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT652.8735632183908044No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA632.7851458885941645No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT522.2988505747126435No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT512.2546419098143233No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT472.0778072502210434No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT381.6799292661361624No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC371.6357206012378427No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT341.5030946065428823No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC301.3262599469496021No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA281.237842617152962No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA231.0167992926613616No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA210.9283819628647214No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG160.707338638373121No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC150.6631299734748011No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC140.618921308576481No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC140.618921308576481No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA110.4862953138815208No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG100.4420866489832007No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT70.3094606542882405No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT60.2652519893899204No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCACGT60.2652519893899204No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC60.2652519893899204No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCNACAACTCCTGTT60.2652519893899204No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCNATTGGGATCATG50.22104332449160036No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCACTTTCAGCTCCATTGGGATCATG50.22104332449160036No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGNGAAGATGCCGAT50.22104332449160036No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGTGGCCCCCTCAGTGA50.22104332449160036No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT50.22104332449160036No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG50.22104332449160036No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCACGC40.17683465959328026No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGT40.17683465959328026No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG40.17683465959328026No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTTCAATCAGCACCCTTTCAACAACTCCTGTT40.17683465959328026No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCTCTGCACCAGGGGAAGCAGTGG40.17683465959328026No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCTGCTCCCCCTGCACCAGGGGAAGCAGTGG30.1326259946949602No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGTAGGACCGCATTGACTACCTGA30.1326259946949602No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGNGCCATTCAGGTA30.1326259946949602No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA30.1326259946949602No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACCTG30.1326259946949602No Hit
CTTCTTGTTTTCGAGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC30.1326259946949602No Hit
ACCTCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA30.1326259946949602No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA30.1326259946949602No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATT30.1326259946949602No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTTCACCACTTCTGCCCCTGCGCACATT30.1326259946949602No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCNACAACTCCTGTT30.1326259946949602No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGATCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG30.1326259946949602No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCACGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA30.1326259946949602No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGNCCCCCTCAGTGA30.1326259946949602No Hit
TCCAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG30.1326259946949602No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCACACACTCGGAAGAAGTAGG30.1326259946949602No Hit
CCTTTAACAAGTATGCCTGTCAGCATCACACCGGTGACCAGTCCTGAGGCTAGCACCCTTTCAACAACTCCTGTT30.1326259946949602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)