FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT222.22222222222222TruSeq Adapter, Index 1 (95% over 21bp)
AACTGTAGCTTACACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAACAA111.11111111111111TruSeq Adapter, Index 1 (96% over 28bp)
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAGAAAAAAA111.11111111111111RNA PCR Primer, Index 1 (96% over 28bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAACTCGTATGCCGTCTT111.11111111111111No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTNGTATGCCGTCTT111.11111111111111No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGCACAAGCTCGTATGCCGTCTT111.11111111111111No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTTTACTATGTGCTTTAGACTCTACACT111.11111111111111No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCACGTATGCCGTCAT111.11111111111111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)