FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30
Sequences flagged as poor quality0
Sequence length75
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG1033.33333333333333No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT310.0No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCGGGTCGCGAAGGGGCCCTG26.666666666666667No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT26.666666666666667No Hit
GCACAGATCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG13.3333333333333335No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAGG13.3333333333333335TruSeq Adapter, Index 8 (96% over 29bp)
CTTCTTGTTTTCGAGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC13.3333333333333335No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCAGCGCGGGGCCCGCGCCCC13.3333333333333335No Hit
CAAGCAATCAAGGCCAGTCTACCCTGAAGCCCTGGCCGCGTTCCCATAAGCAGCACGCTGCACAGCATCATCCAT13.3333333333333335No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG13.3333333333333335No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTCCCTGA13.3333333333333335No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCAC13.3333333333333335No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGTACCGCCTGGCAGGTCGCGAAGGGGCCCTG13.3333333333333335No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGTGCCCCCTCAGTGA13.3333333333333335No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA13.3333333333333335No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGTA13.3333333333333335TruSeq Adapter, Index 10 (96% over 28bp)
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC13.3333333333333335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)