FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG824.242424242424242No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT26.0606060606060606No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA26.0606060606060606No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC26.0606060606060606No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA13.0303030303030303No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGNGGCCCGCGCCCC13.0303030303030303No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTACCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG13.0303030303030303No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGAGCCCTG13.0303030303030303No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA13.0303030303030303No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAGTCAGCACCCTTTCAACAACTCCTGTT13.0303030303030303No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGAAATCAGCACCCTTGCAAGAACGCCTGTT13.0303030303030303No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGAACCGCATTGACTACCTGA13.0303030303030303No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGTACTCAGCACCGCCTGGCAGGTCTCGAAGGGGCCCTG13.0303030303030303No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGTGGGCCCCCTCAGTGA13.0303030303030303No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG13.0303030303030303No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCACTGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG13.0303030303030303No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGACAGGTCGNGAAGGGGCACTG13.0303030303030303No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT13.0303030303030303No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTACAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC13.0303030303030303No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCNATTGGGATCATG13.0303030303030303No Hit
GCTCTCAGCTCCGAGCCCACGAGACAGGCAGAAAGCTCGTATGCCGTCTTCTGCTTGAAAACAAAAAAGTAAAAT13.0303030303030303Illumina Single End Adapter 1 (96% over 26bp)
GTAGTGCTGCTCGTACAGGTAGCAGGCAACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT13.0303030303030303No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)